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KMID : 1025720120310010020
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Journal of Dental Implant Research
2012 Volume.31 No. 1 p.20 ~ p.25
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Comparative study of mineralization methods for making human demineralized dentin matrix - comparison 2% HNO3 and 0.6 N HCl -
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Hwang Hong-Jun
Kim Yeong-Wook
Mo dong-Yub
Lee Jang-Yeol
Kim Hyun-Chull
Lee Sang-Chull
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Abstract
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Purpose:The aim of this study is finding more efficient demineralizing method through comparision 2% HNO3 and 0.6 N HCl.
Materials and Methods: A human demineralized dentin matrix (DDM) was prepared from extracted molars. The molars were particulated and its size varied from 0.5 to 1 mm. The particles were demineralized by 2% HNO3 or 0.6 N HCl. The weights of particles were measured and the radiographs were taken before and after the demineralization. And also decrementof inorganic matter was analyzed.
Results: Mean weight loss was 52.5%, 45% in HNO3, HCl, respectively. Radiographically, the image of the particles in HNO3 lost more radiopacity than the particles in HCl. 2% HNO3 showed further reduction of inorganic components (Ca, P) more than 0.6 N HCl.
Conclusion: This result showed that HNO3 is more efficient material than HCl for tooth demineralization. Further long term evaluation via prospectively designed studies are needed.
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KEYWORD
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Autogenous demineralized dentin matrix, 2% HNO3, 0.6 N HCl
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